A. Summary of Dr. Srivastava
B. Special Stem Cell Issues of Journals Devoted to Dr. Srivastava
C. Dr. Srivastava’s Major Scientific Achievements
D. Positions Held by Dr. Srivastava (1997 to date)
E. Dr. Srivastava's Expertise as Scientific Reviewer for Leading Medical Journals
F. Fellowships and Awards Received by Dr. Srivastava
G. Grants and Award Funding Related to Dr. Anand Srivastava’s Work
H. Dr. Srivastava’s Excellence In Several Advanced Biological Techniques
I. Speaker Invitations and Presentations of Dr. Srivastava’s Scientific Findings in National and International Conferences
J. Scientific Publications (More than 50 in Total)
Dr. Anand Srivastava has been associated with leading universities and research institutions of USA. In affiliation with University of California San Diego Medical College (UCSD), University of California Irvine Medical College (UCI), Salk Research Institute, San Diego, Burnham Institute for Medical Research, San Diego, University of California Los Angeles Medical College (UCLA), USA, Dr. Srivastava has developed several research projects. He has extensive research experience in the field of stem cells, which is documented by several publications in revered scientific journals.
Dr. Anand Srivastava’s success is rooted in his unique background and expertise in stem cell biology, protein biochemistry, molecular biology, immunology, in utero transplantation of stem cells, tissue targeting, gene therapy, and clinical research. There are many scientists who can work in a narrowly defined field, but few have the broad and multidisciplinary experience to carry out clinical research in fields as challenging as stem cell biology, cancer and gene therapy. Dr. Anand Srivastava’s wide-spectrum expertise is rare in clinical research, and perfectly crafted to fit ideally with GIOSTAR projects for stem cell transplantation, cancer and gene therapy research.
Dr. Anand Srivastava’s research work has been presented in various national and international scientific meetings and conferences in India, Japan, Germany and USA. His research articles have been published in peer-reviewed medical scientific journals, and his work has been cited extensively by other scientists. Dr. Anand Srivastava’s expertise and scientific achievements have been recognized by many scientific fellowships, and his being awarded by the prestigious and internationally recognized JAPAN International Science and Technology Exchange Center (JISTEC) Award from the Government of JAPAN. Also, he received the "excellent presentation award" in the Meeting of Clinical Chemistry and Medicine in Kyoto, JAPAN.
Dr. Srivastava has also expertise in genetic engineering research, developmental biology, immunology, making of transgenic animals. His extraordinary expertise in searching and characterizing new genes are ideal for our ongoing projects of developing effective treatments for many degenerative diseases, genetic diseases and cancer. Based on his extraordinary scientific achievements, his biography has been included in the "Who's Who in America" 2018 data bank twice, first in 2005 and second in 2010. He has received the USA Congressional Recognition for his contribution in the field of stem cell science.
Dr. Srivastava is a Chairman and Cofounder of the California-based Global Institute of Stem Cell Therapy and Research (GIOSTAR) headquartered in San Diego, California (USA). The company was formed with the vision to provide stem cell based therapy to aid those suffering from degenerative or genetic diseases around the world such as Parkinson’s disease, Alzheimer’s disease, Autism, Diabetes, Heart Disease, Stroke, Spinal Cord Injuries, Paralysis, Blood Related Diseases, Cancer, and Burns. GIOSTAR is a leader in developing most advanced stem cell based technology, supported by leading scientists with pioneering publications in the area of stem cell biology. The company’s primary focus is to discover and develop a cure for human diseases, with state-of-the-art, unique stem cell based therapies and products. Regenerative medicine provides promise for treatments of diseases previously regarded as incurable.
For over a decade, GIOSTAR has been the world’s leading company involved in stem cell research work. The firm is headed by Dr. Anand Srivastava, who is a world-renowned authority in the field of stem cell biology, cancer and gene therapy. Several governments and organizations - including USA, India, China, Turkey, Kuwait, Thailand, Philippines, Bahamas, Saudi Arabia and many others - seek his advice and guidance on drafting their strategic and national policy formulations and program directions in the area of stem cell research, development, and its regulations.
Under Dr. Srivastava's creative leadership, a group of esteemed scientists and clinicians have developed and established stem cell therapy for various types of autoimmune diseases and blood disorders, which are being offered to patients in USA. The therapy will soon be offered on a regular clinical basis to people around the globe.
GIOSTAR is already the official collaborator of Government of Gujarat, India by setting up a state of art stem cell treatment hospital in Surat civil hospital for the less fortunate tribal populace of the southern belt of Gujarat suffering from sickle cell anemia. Several state governments in India are looking seeking to collaborate with GIOSTAR and Dr. Anand in their efforts to develop stem cell transplantation programs in their respective states.
Certificate of Recognition
Additionally, Dr. Srivastava has contributed to stem cell programs, and has guided stem cell policies and programs for following governments and private organizations:
Dr. Srivastava is a member of several scientific review committees, and is closely involved in reviewing research grants. He has written several review articles and scientific manuscripts. He is also the reviewer of several scientific journals, including:
Awarded with “National Integrated Medical Association (NIMA) Outstanding Scientist” award from NIMA, India.
Awarded with “Excellent Scientist Award” from Bharat Vikas Parisad, India for continuous excellent performance in the life science research.
The 18th International Congress of Clinical Chemistry and Laboratory Medicine Kyoto “Excellent Poster Award,” Kyoto, JAPAN.
“Best Scientist Award” for excellent contribution in the field of life science research from Kayastha Maha Sabha, Varanasi, India.
“Long-term STA/JISTEC Award” (Science and Technology Agency/Japan International Science and Technology Exchange Center, Japan)
Fellowship award for two years from government of JAPAN.
“Short-term STA/JISTEC Award” (Science and Technology Agency/Japan International Science and Technology Exchange Center, Japan)
Fellowship award for three months from government of JAPAN (October 1997- January 1998).
Awarded with “Research Associate-Ship Award” from Council of Scientific and Industrial Research (CSIR) Government of India.
“CAS (Center of Advanced Study) Award” in Zoology.
Doctoral research fellowship award from Government of India.
Techniques related to Human Embryonic Stem Cell
Human Embryonic Stem cell culture, Serum free and feeder free hES cell culture, in vitro differentiation of hES cells into neural cells, in vitro differentiation of hES into hematopoietic cells and red blood cells under the control of cytokines. Gene regulation studies using RT-PCR, Real time PCR, Northern blot, Southern blot and in situ hybridization, immunohistochemistry during the differentiation, Cell cycle regulation studies during differentiation of hES cells into hematopoietic and neural cells. Use of siRNA for blocking a specific cell cycle. FACS analysis of differentiated cells and cell shorting. ES cell transfection.
In vivo studies with ES cells
Created a mouse model for study the effect of ES cells on damaged brain. Injection of ES cells into mouse brain, tail vein injection, in vivo tracking of ES cell migration. Used the ES cells for repair of damaged brain. Gene and protein regulation during neural cell differentiation. Studies on transcription factors. Histochemical analysis of transplanted ES cells using fluorescent, confocal microscopy and deconvolution microscopy.
Created a mouse model for Crohn’s disease. In vivo migration of ES cells into diseased portion of intestine. Studies on inflammatory cytokines during the repair of Crohn’s disease with ES cell. Gene regulation studies during this process. Elisa assays for the cytokines. Stem cell niche interaction.
Created in utero mouse model for ES cells transplantation. Used this model to make chimeric animals. Distribution and differentiation of ES cells into developing mouse embryo. FACS and magnetic shorting of ES cells derived CD31+, CD34+, CD45+ cells from the transplanted animal tissues. Gene and protein regulation of in vivo differentiating cells.
Created immunocompromised mouse model to study the effect of in vivo immune component on T7 phage virus. In vivo selection of tissue specific receptor binding peptide using in vivo biopanning method. Tissue targeted gene delivery to correct the blood related genetic diseases. Gene cloning, gene sequencing, synthesis of RNA probes.
Protein and enzyme biochemistry
Protein assay, peptide structure and amino acid sequencing, Enzyme assay, Ultra centrifugation, Ion exchange chromatography, column chromatography, HPLC, Protein and gene regulation during the development. Enzyme kinetics, Enzyme inhibition, SDS gel electrophoresis, Protein characterization.
Selection of cell receptor binding peptide and Phage display technology
Fluorescence techniques for nucleic acid sequence detection: clinical and diagnostic applications
Gene Detection Technology: Research and Clinical Applications
Standard molecular biology techniques
General molecular and biochemical techniques
mRNA preparation and purification, Primer designing, Real-time PCR, RT-PCR, DNA cloning, DNA sequencing, Isolation of Genomic DNA, Genomic library Construction, Transformation, Transfection, Cell culture, Plasmid purification, RNA probe making, Different kinds of microscopy, In situ hybridization, Southern blotting, Northern blotting, Western blotting, Spectrophotometery, In utero microinjection, Column chromatography, HPLC, PAGE, Agarose gel-electrophoresis, Enzyme assay, Protein assay, Enzyme/ Protein/ DNA purification, Histology, Phage display for tissue targeting, Radio-immunoassay.
Autologous stem cell transplant techniques
Developed the techniques to isolate the autologous stem cell from patient’s peripheral blood, bone marrow or fat tissues, characterize and activate those stem cells so they can be effectively used for the treatment of specific diseases.
48. Suzuki T., Srivastava A.S., Kurokawa T.; Japanese flounder mRNA for trypsinogen 2. Cent. Biotech. Infor., USA, AB029751, (1999).
49. Suzuki T., Srivastava A.S., Kurokawa T.; Japanese flounder mRNA for trypsinogen 3. Cent. Biotech. Infor., USA, AB029752, (1999).
50. Suzuki T., Srivastava A.S., Kurokawa T.; Japanese flounder mRNA for chymotrypsinogen 1. Cent. Biotech. Infor., USA, AB029753 (1999).
51. Suzuki T., Srivastava A.S., Kurokawa T.; Japanese flounder mRNA for chymotrypsinogen 2. Cent. Biotech. Infor., USA, AB029754 (1999).
52. Suzuki T., Srivastava A.S., Kurokawa T.; Japanese flounder mRNA for elastase 1 precursor. Cent. Biotech. Infor., USA, AB029755, (1999).
53. Suzuki T., Srivastava A.S., Kurokawa T.; Japanese flounder mRNA for elastase 2 precursor. Cent. Biotech. Infor., USA, AB029756, (1999).
54. Suzuki T., Srivastava A.S., Kurokawa T.; Japanese flounder mRNA for elastase 3 precursor. Cent. Biotech. Infor., AB029757, (1999).
55. Suzuki T., Srivastava A.S., Kurokawa T.; Japanese flounder mRNA for elastase 4 precursor. Cent. Biotech. Infor., USA, AB029758, (1999).
56. Srivastava A.S., Oohara I., Suzuki T., Singh S.N.; Activity and expression of aspartate aminotransferase during reproductive cycle of a fresh water fish, Clarias batrachus. Fish Physiol. Biochem., 20:243-250,1999.
57. Srivastava A.S., Oohara I., Suzuki T., Singh S.N.; Activity and expression of Glutamate Oxaloacetate Transaminase during reproductive cycle of a fresh water fish Labeo rohita. Fisheries Science, 64(4):621-626,1998.
58. Srivastava A.S., Trigun S.K., Singh S.N.; Activity of cytosolic and mitochondrial alanin aminotransferase during pre-spawning phase in air-breathing and non air-breathing fishes. J. Sci. Res., 47:33-38,1997.
Anand S. Srivastava, MS, PhD
Chairman & Cofounder of GIOSTAR
Dr. Anand Srivastava is a proud recipient of the United States House of Representatives Certificate of Recognition for "Best Of Business" in recognition for co-founding GlOSTAR
Dr. Anand Srivastava cloned 23 genes while working for the government of Japan in 1998. In 1997 and 1998, he was awarded the Japan International Science and Technology Exchange Center (JISTEC) Award, a highly prestigious international scientific award. He is the only leading scientist to have earned this accolade twice. Dr. Srivastava also earned the Who’s Who award in 2005, 2010, 2014, and 2018.
Working at UC Irvine, Dr. Srivastava and the team of researchers conducted the first FDA Approved Human Clinical Trial with the Christopher & Dana Reeve Foundation for spinal cord injury, which started in 2007.
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